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| Qing,Chang; Wei-ding,Cui; Wei-min,Fan. |
| Chondrocytes and bone marrow mesenchymal stem cells (BMSCs) are frequently used as seed cells in cartilage tissue engineering. In the present study, we determined if the co-culture of rabbit articular chondrocytes and BMSCs in vitro promotes the expression of cartilaginous extracellular matrix and, if so, what is the optimal ratio of the two cell types. Cultures of rabbit articular chondrocytes and BMSCs were expanded in vitro and then cultured individually or at a chondrocyte:BMSC ratio of 4:1, 2:1, 1:1, 1:2, 1:4 for 21 days and cultured in DMEM/F12. BMSCs were cultured in chondrogenic induction medium. Quantitative real-time RT-PCR and Western blot were used to evaluate gene expression. In the co-cultures, type II collagen and aggrecan expression... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Co-culture; Bone mesenchymal stem cells; Chondrocytes; Tissue engineering. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400006 |
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| Fakheri,Taravat; Nankali,Anisodowleh; Khazaei,Mozafar; Ghanbari,Ali; Abedini,Mehrandokht; Saeidiborojeni,Hamidreza. |
| There were no significant differences in the distribution of embryos reaching to 2- cells, 4- cells, morula or blastocysts culturing on human endometrial stromal cells (Secretory or proliferative phases). The percent of morula in stage A (without fragmentation), stage B (<25% fragmentation), stage C (25-50% fragmentation) and stage D (>50% fragmentation) and did not showed significant differences between two coculture groups. Thus, the phase that the endometrial stromal cells were in thereby did not affect on the quality of embryos. |
| Tipo: Journal article |
Palavras-chave: Co-culture; Embryo; Endometrium; Mice; Proliferative phase; Secretory phase. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400014 |
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